Advances in Immunology, Vol. 33 by Henry G. Kunkel, Frank J. Dixon (Eds.)

By Henry G. Kunkel, Frank J. Dixon (Eds.)

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These findings demonstrate that F, male B cells have a defect in their ability to respond to the T D antigen SRBC in the presence of T cells which provide sufficient help to F, female B cells. The capacity of the F, male B cells to respond to SRBC was considerably improved if the B cells were derived from mice that had been primed to SRBC. Different results were obtained by E. Metcalf et al. (1980) who demonstrated that the IgM and IgG anti-TNP responses of these mice to TNP-KLH were equivalent to normals.

I a 0 100 I w 3 - a I I 0 I I 103 Dose 5. 5. Each point represents the percentage of five (CBNN x DBA/B)F, male mice that were dead at 10 (0)and 28 (A) days postinfection with S . typhimurium. , 1979). the mechanism(s) responsible for the control of the bacteria early in the course of the infection was similar in these mice, but that the Fl male mice were defective in controlling the multiplication of the S. typhimurium later in the infection. This was supported by the finding that F, female anti-S.

B-CELL COLONYFORMATION Clusters and colonies of up to 3000 cells develop in semisolid agar when murine spleen, lymph node, peritoneal cavity, thoracic duct, or blood cells are plated under appropriate conditions (D. , 1975). Important for the growth of these cells are mitogens, including LPS or other stimulatory factors, which can be supplied by SRBC, 0-mercaptoethanol (D. , 1976). The cells formed in the clusters or colonies bear Fc receptors; up to approximately 70% react with anti-p antibodies, and their morphology is that of immature lymphoid and plasma cells (D.

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